TNF alpha Monoclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-22159-60, E-AB-22159-120, E-AB-22159-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: TNF α

Target Synonym: APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily; member 2; TNF; macrophage derived; TNF; monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA; TNFSF2; Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor; Membrane Form; Tumor necrosis factor; soluble form

Research Areas: Cancer, Cardiovascular, Metabolism, Immunology, Signal Transduction

Conjugation: Unconjugated

Host: Mouse

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC-p

Isotype: IgG

Clonality: Monoclonal

Clone No.: Q34

UNIProt ID: P01375

Background: TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Concentration: 1 mg/mL

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Protein A purification

Dilution: WB 1:1000-1:3000, IHC 1:50-1:200

Observed MW: 17,25kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only