HVA (Homovanillic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7921-48, ELK7921-96, ELK7921-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HOC6H3 (OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

Assay Type: Competitive Inhibition

Sensitivity: 0.96 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only