Histone H2A.X Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12063-50, MB12063-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P16104

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of Histone H2A.X in Hela using Histone H2A.X antibody, and DAPI., Western blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X

Immunogen: A synthetic peptide of human Histone H2A.X

Conjugate: Unconjugated

Modification: Unmodified