Phospho-AKT1 (Ser473) Monoclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-51038-60, E-AB-51038-120, E-AB-51038-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Phospho-AKT1 (Ser473)

Target Synonym: AKT 1; AKT; AKT1; AKT1; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha

Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Mouse

Species Reactivity: Human

Application: WB, IHC-p

Isotype: IgG

Clonality: Monoclonal

Clone No.: Clone:7F9

UNIProt ID: P31749

Background: The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Concentration: 1 mg/mL

Immunogen: Synthetic Peptide of Phospho-Akt (S473)

Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Purification Method: Protein A purification

Dilution: WB 1:1000-2000, IHC 1:100-200

Observed MW: 60kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only